Translational_Unit

Part:BBa_K4310011:Design

Designed by: Seungchan Oh   Group: iGEM22_UIncheon   (2022-10-11)


constitutively expressed mCherry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 837
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 837
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 226
    Illegal BglII site found at 1206
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 837
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 837
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1388
    Illegal BsaI.rc site found at 1667
    Illegal SapI site found at 439
    Illegal SapI.rc site found at 473


Design Notes

CAMV 35S: BBa_J428074 from distribution 2022 Kozak sequence: BBa_K4310003 mCherry: BBa_J428079 from distribution 2022 Tnos: BBa_J428082 from distribution 2022


Source

To make compatible to our assembly, CAMV 35S, mCherry and Tnos are amplified by overhang primers.

References